DNA constraints allow rational control of macromolecular conformation.

Synthesis and application of a 5'-aldehyde phosphoramidite for covalent attachment of DNA to biomolecules.

We recently reported the use of covalently attached DNA as a structural constraint for rational control of macromolecular conformation. Reductive amination was employed to attach each strand of the duplex DNA constraint to RNA, utilizing an aldehyde tethered to the 5'-terminus of the DNA. Here we describe the synthesis of a thymidine phosphoramidite that has the 5'-tethered aldehyde masked as a...

Control of macromolecular structure and function using covalently attached double-stranded DNA constraints.

The biophysical properties of DNA suggest its use for applications beyond serving as the genetic material. Several recent reports describe the use of covalently attached double-stranded DNA for controlling the structures of other macromolecules such as protein and RNA. These exploitations of DNA rigidity are conceptually distinct from many other studies in the area of "DNA nanotechnology". Doub...

Modulation of DNA constraints that control macromolecular folding.

Efficient control of group I intron ribozyme catalysis by DNA constraintsw

Rational attachment of double-stranded DNA as a structural constraint allows control over macromolecular conformation and function. Others have modulated the structure or enzymatic activity of a protein by changing the hybridization state of a DNA strand that is attached at both ends to the protein. We used DNA constraints to control the tertiary structure of the 160-nucleotide P4–P6 RNA domain...

X-ray solution scattering (SAXS) combined with crystallography and computation: defining accurate macromolecular structures, conformations and assemblies in solution.

Crystallography supplies unparalleled detail on structural information critical for mechanistic analyses; however, it is restricted to describing low energy conformations of macromolecules within crystal lattices. Small angle X-ray scattering (SAXS) offers complementary information about macromolecular folding, unfolding, aggregation, extended conformations, flexibly linked domains, shape, conf...